TRPM2 channel contribution to CdCI2-related neurotoxicity in SH-SY5Y cells: Protective role of selenium
Effect of Se in CdCI2-related neurotoxicity
DOI:
https://doi.org/10.5281/zenodo.13623107Anahtar Kelimeler:
Cadmium, Selenium, SH-SY5Y cells, TRPM2 channelÖz
Humans are most exposed to the heavy metal cadmium (Cd), known as neurotoxic. However, it is unclear how selenium (Se) protects neurons from damage caused by increased Cd-induced neurotoxicity in SH-SY5Y cells and how the TRPM2 channel functions in this process. In this study, we examined the impact of Se on CdCI2-induced oxidative neurotoxicity and cell death in SH-SY5Y cells by modifying the TRPM2 channel. The Se and TRPM2 channel antagonist 2-APB was added to prevent CdCI2-induced neurotoxicity in SH-SY5Y cells. Cell viability rate was determined between groups by CCK-8 assay. GSH, MDA, and ROS levels were determined in the cells with ELISA kits. Our results showed that the TRPM2 channel plays a vital role in forming CdCI2-induced damage to cells by using the TRPM2 antagonist in the study. We also observed that Se reduced CdCI2-induced neurotoxicity by reducing TRPM2 channel activation by suppressing oxidative stress of cells. We conclude that Se therapy and TRPM2 channel blocking can reduce CdCI2-induced neurotoxicity based on our investigation, which examined the protective impact of Se and the involvement of the TRPM2 channel in CdCI2-induced SH-SY5Y cells for the first time.
İndirmeler
Referanslar
Bernhoft RA, Cadmium toxicity and treatment. ScientificWorldJournal. 2013;2013:394652. https://doi.org/10.1155/2013/394652
Fatima G, Raza AM, Hadi N, et al. Cadmium in human diseases: It’s more than just a mere metal. Indian Journal of Clinical Biochemistry. 2019;34:371-78. https://doi.org/10.1007/s12291-019-00839-8
Aljelehawy QHA, Effects of the lead, cadmium, manganese heavy metals, and magnesium oxide nanoparticles on nerve cell function in Alzheimer’s and Parkinson’s diseases. Cent. Asian J. Med. Pharm. Sci. Innov. 2022;2: 25-36. https://doi.org/10.22034/CAJMPSI.2022.01.04
Forcella M, Lau P, Oldani M, et al. Neuronal specific and non-specific responses to cadmium possibly involved in neurodegeneration: A toxicogenomics study in a human neuronal cell model. Neurotoxicology. 2020;76:162-73. https://doi.org/10.1016/j.neuro.2019.11.002
Shayan M, Mehri S, Razavi BM, et al. Minocycline protects PC12 cells against cadmium-induced neurotoxicity by modulating apoptosis. Biological Trace Element Research. 2023;201(4):1946-54. https://doi.org/10.1007/s12011-022-03305-4
Al Olayan EM, Aloufi AS, AlAmri OD, et al. Protocatechuic acid mitigates cadmium-induced neurotoxicity in rats: Role of oxidative stress, inflammation and apoptosis. Science of the total environment. 2020;723: 137969. https://doi.org/10.1016/j.scitotenv.2020.137969
Ren TT, Yang JY, Wang J, et al. Gisenoside Rg1 attenuates cadmium-induced neurotoxicity in vitro and in vivo by attenuating oxidative stress and inflammation. Inflammation Research. 2021;70:1151-64. https://doi.org/10.1007/s00011-021-01513-7
Yıldız MO, Çelik H, Caglayan C, et al. Neuroprotective effects of carvacrol against cadmium-induced neurotoxicity in rats: role of oxidative stress, inflammation, and apoptosis. Metabolic brain disease. 2022;37(4):1259-69. https://doi.org/10.1007/s11011-022-00945-2
Malko P and Jiang LH. TRPM2 channel-mediated cell death: An important mechanism linking oxidative stress-inducing pathological factors to associated pathological conditions. Redox Biol. 2020;37:101755. https://doi.org/10.1016/j.redox.2020.101755
Yıldızhan K and Nazıroğlu M. NMDA receptor activation stimulates hypoxia-induced TRPM2 channel activation, mitochondrial oxidative stress, and apoptosis in neuronal cell line: Modular role of memantine. Brain Research. 2023:148232. https://doi.org/10.1016/j.brainres.2023.148232
Ahlatcı A, Yıldızhan K, Tülüce Y, Bektaş M. Valproic acid attenuated PTZ-induced oxidative stress, inflammation, and apoptosis in the SH-SY5Y cells via modulating the TRPM2 channel. Neurotoxicity Research. 2022;40(6):1979-88. https://doi.org/10.1007/s12640-022-00622-3
Shi R, Fu Y, Zhao D, et al. Cell death modulation by transient receptor potential melastatin channels TRPM2 and TRPM7 and their underlying molecular mechanisms. Biochemical Pharmacology 2021;190:114664. https://doi.org/10.1016/j.bcp.2021.114664
An X, Fu Z, Mai C, et al. Increasing the TRPM2 channel expression in human neuroblastoma SH-SY5Y cells augments the susceptibility to ROS-induced cell death. Cells. 2019;8(1):28. https://doi.org/10.3390/cells8010028
Naziroglu M, Oz A, Yildizhan K. Selenium and neurological diseases: Focus on peripheral pain and TRP channels. Curr Neuropharmacol. 2020;18(6):501-17. https://doi.org/10.2174/1570159X18666200106152631
Avery JC and Hoffmann PR. Selenium, selenoproteins, and immunity. Nutrients. 2018;10(9). https://doi.org/10.3390/nu10091203
Branca JJ, Morucci G, Maresca M, et al. Selenium and zinc: Two key players against cadmium-induced neuronal toxicity. Toxicology in Vitro 2018;48:159-69. https://doi.org/10.1016/j.tiv.2018.01.007
Al Kahtani M. Effect of both selenium and biosynthesized nanoselenium particles on cadmium-induced neurotoxicity in albino rats. Human & Experimental Toxicology. 2020;39(2):159-72. https://doi.org/10.1177/0960327119880589
Yıldızhan K and Nazıroğlu M. Protective role of selenium on MPP+ and homocysteine-induced TRPM2 channel activation in SH-SY5Y cells. Journal of Receptors and Signal Transduction. 2022;42(4):399-408. https://doi.org/10.1080/10799893.2021.1981381
Ertilav K, Nazıroğlu M, Ataizi ZS, et al. Selenium enhances the apoptotic efficacy of docetaxel through activation of TRPM2 channel in DBTRG glioblastoma cells. Neurotoxicity research. 2019;35:797-808. https://doi.org/10.1007/s12640-019-0009-5
Branca JJ, Fiorillo C, Carrino D, et al. Cadmium-induced oxidative stress: focus on the central nervous system. Antioxidants. 2020;9(6):492. https://doi.org/10.3390/antiox9060492
Patra R, Rautray AK, Swarup D. Oxidative stress in lead and cadmium toxicity and its amelioration. Veterinary Medicine International. 2011;2011. https://doi.org/10.4061/2011/457327
Chen L, Liu L, Luo Y, et al. MAPK and mTOR pathways are involved in cadmium‐induced neuronal apoptosis. Journal of Neurochemistry 2008;105(1):251-61. https://doi.org/10.1111/j.1471-4159.2007.05133.x
Mallamaci R, Storelli MM, Barbarossa A, et al. Potential protective effects of spirulina (spirulina platensis) against in vitro toxicity induced by heavy metals (cadmium, mercury, and lead) on SH-SY5Y neuroblastoma cells. International Journal of Molecular Sciences. 2023;24(23):170-76. https://doi.org/10.3390/ijms242317076
Wang H, Sun S, Ren Y, et al. Selenite ameliorates cadmium-induced cytotoxicity through downregulation of ROS levels and upregulation of selenoprotein thioredoxin reductase 1 in SH-SY5Y cells. Biological Trace Element Research. 2023;201(1):139-48. https://doi.org/10.1007/s12011-022-03117-6
Chen S, Ren Q, Zhang J, et al. N‐acetyl‐L‐cysteine protects against cadmium‐induced neuronal apoptosis by inhibiting ROS‐dependent activation of A kt/m TOR pathway in mouse brain. Neuropathology and applied neurobiology. 2014;40(6):759-77. https://doi.org/10.1111/nan.12103
Ahlatci A. Investıgatıon of The Amelıoratıve Effects of Gallic Acid Agaınst Neurotoxicity Caused by Glutamate in C6 Cells: Effect of gallic acid on Glutamate-Induced neurotoxicity. Neuro-Cell Mol Res. 2024;1(1):7-13. https://doi.org/10.5281/zenodo.11471554
Shati AA. Resveratrol protects against cadmium chloride‐induced hippocampal neurotoxicity by inhibiting ER stress and GAAD 153 and activating sirtuin 1/AMPK/Akt. Environmental toxicology. 2019;34(12):1340-53. https://doi.org/10.1002/tox.22835
Yayınlanmış
Nasıl Atıf Yapılır
