Hesperidin protects against MPP+-induced neurotoxicity in SH-SY5Y cells
DOI:
https://doi.org/10.5281/zenodo.15311443Anahtar Kelimeler:
Hesperidin- Parkinson’s disease- Cytotoxicity- Oxidative stress- SH-SY5Y cellsÖz
Dopaminergic neuron loss and alpha-synuclein aggregation brought on by an excess of reactive oxygen species (ROS) are linked to Parkinson's disease (PD). The pharmacological characteristics of hesperidin (HES), including its anti-inflammatory, anti-atherosclerosis, anti-diabetic, anti-cancer effects have been the subject of much recent research. However, its possible neuroprotective effects on SH-SY5Y cells, a cellular PD model, caused by the neurotoxic MPP+, have not been studied. In this investigation, we postulated that antioxidant activities may scavenge ROS, hence mediating neuroprotective benefits against MPP+-induced oxidative stress parameters. Here, HES pre-treatment could attenuate MPP+-induced neuronal cell death, which was reflected by decreasing the levels of ROS, Total Oxidant Status (TOS) and inflammation. Moreover, HES led to increased levels of the antioxidant enzyme GSH and TOS. According to this study, HES demonstrated notable neuroprotective benefits against MPP+-induced SH-SY5Y cell death in vitro. Our findings demonstrate that HES may be a valuable adjunct for Parkinson's disease prevention.
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Chi H, Chang HY, Sang TK. Neuronal Cell Death Mechanisms in Major Neurodegenerative Diseases. Int J Mol Sci. 2018;19(10):3082. https://doi.org/10.3390/ijms19103082
Van Schependom J, D’haeseleer M. Advances in neurodegenerative diseases. MDPI. 2023;1709 https://doi.org/10.3390/jcm12051709
Zeng R, Wang J, Zheng C, et al. Lack of Causal Associations of Inflammatory Bowel Disease with Parkinson's Disease and Other Neurodegenerative Disorders. Mov Disord. 2023;38(6):1082-1088. https://doi.org/10.1002/mds.29386
Morris HR, Spillantini MG, Sue CM, Williams-Gray CH. The pathogenesis of Parkinson's disease. Lancet. 2024;403(10423):293-304. https://doi.org/10.1016/S0140-6736(23)01478-2
Gong P, Deng F, Zhang W, et al. Tectorigenin attenuates the MPP+-induced SH-SY5Y cell damage, indicating a potential beneficial role in Parkinson's disease by oxidative stress inhibition. Exp Ther Med. 2017;14(5):4431-4437. https://doi.org/10.3892/etm.2017.5049
Yıldızhan K, Nazıroğlu M. Protective role of selenium on MPP+ and homocysteine-induced TRPM2 channel activation in SH-SY5Y cells. J Recept Signal Transduct Res. 2022;42(4):399-408. https://doi.org/10.1080/10799893.2021.1981381
Zaim M, Kara I, Muduroglu A. Black carrot anthocyanins exhibit neuroprotective effects against MPP+ induced cell death and cytotoxicity via inhibition of oxidative stress mediated apoptosis. Cytotechnology. 2021;73(6):827-840. https://doi.org/10.1007/s10616-021-00500-4
Wang Y, Gao J, Miao Y, et al. Pinocembrin protects SH-SY5Y cells against MPP+-induced neurotoxicity through the mitochondrial apoptotic pathway. J Mol Neurosci. 2014;53(4):537-545. https://doi.org/10.1007/s12031-013-0219-x
Ramalingam M, Kim SJ. The Neuroprotective Role of Insulin Against MPP(+) -Induced Parkinson's Disease in Differentiated SH-SY5Y Cells. J Cell Biochem. 2016;117(4):917-926. https://doi.org/10.1002/jcb.25376
Song Q, Peng S, Zhu X. Baicalein protects against MPP+/MPTP-induced neurotoxicity by ameliorating oxidative stress in SH-SY5Y cells and mouse model of Parkinson's disease. Neurotoxicology. 2021;87:188-194. https://doi.org/10.1016/j.neuro.2021.10.003
Aboraya DM, El Baz A, Risha EF, Abdelhamid FM. Hesperidin ameliorates cisplatin induced hepatotoxicity and attenuates oxidative damage, cell apoptosis, and inflammation in rats. Saudi J Biol Sci. 2022;29(5):3157-3166. https://doi.org/10.1016/j.sjbs.2022.01.052
Bayir MH, Yıldızhan K, Altındağ F. Effect of Hesperidin on Sciatic Nerve Damage in STZ-Induced Diabetic Neuropathy: Modulation of TRPM2 Channel. Neurotox Res. 2023;41(6):638-647. https://doi.org/10.1007/s12640-023-00657-0
Liu WY, Liou SS, Hong TY, Liu IM. Protective Effects of Hesperidin (Citrus Flavonone) on High Glucose Induced Oxidative Stress and Apoptosis in a Cellular Model for Diabetic Retinopathy. Nutrients. 2017;9(12):1312. Published 2017 Dec 2. https://doi.org/10.3390/nu9121312
Hanchang W, Khamchan A, Wongmanee N, Seedadee C. Hesperidin ameliorates pancreatic β-cell dysfunction and apoptosis in streptozotocin-induced diabetic rat model. Life Sci. 2019;235:116858. https://doi.org/10.1016/j.lfs.2019.116858
Kesh S, Kannan RR, Sivaji K, Balakrishnan A. Hesperidin downregulates kinases lrrk2 and gsk3β in a 6-OHDA induced Parkinson's disease model. Neurosci Lett. 2021;740:135426. https://doi.org/10.1016/j.neulet.2020.135426
Çınar R, Yıldızhan K. Curcumin protects against MPP+-induced neurotoxicity in SH-SY5Y cells by modulating the TRPV4 channel. Mol Biol Rep. 2025;52(1):255. https://doi.org/10.1007/s11033-025-10345-1
Lim C, Zhen AX, Ok S, et al. Hesperidin Protects SH-SY5Y Neuronal Cells against High Glucose-Induced Apoptosis via Regulation of MAPK Signaling. Antioxidants (Basel). 2022;11(9):1707. https://doi.org/10.3390/antiox11091707
Agod Z, Fekete T, Budai MM, et al. Regulation of type I interferon responses by mitochondria-derived reactive oxygen species in plasmacytoid dendritic cells. Redox Biol. 2017;13:633-645. https://doi.org/10.1016/j.redox.2017.07.016
Rubio-Osornio M, Orozco-Ibarra M, Díaz-Ruiz A, et al. Copper sulfate pretreatment prevents mitochondrial electron transport chain damage and apoptosis against MPP+-induced neurotoxicity. Chem Biol Interact. 2017;271:1-8. https://doi.org/10.1016/j.cbi.2017.04.016
Yan J, Ma H, Lai X, et al. Artemisinin attenuated oxidative stress and apoptosis by inhibiting autophagy in MPP+-treated SH-SY5Y cells. J Biol Res (Thessalon). 2021;28(1):6. https://doi.org/10.1186/s40709-021-00137-6
Liu M, Zuo S, Guo X, et al. The Study of Overexpression of Peroxiredoxin-2 Reduces MPP+-Induced Toxicity in the Cell Model of Parkinson's Disease. Neurochem Res. 2023;48(7):2129-2137. https://doi.org/10.1007/s11064-023-03880-5
Ahlatci A. Investıgatıon of The Amelıoratıve Effects of Gallic Acid Agaınst Neurotoxicity Caused by Glutamate in C6 Cells: Effect of gallic acid on Glutamate-Induced neurotoxicity. Neuro-Cell Mol Res. 2024;1(1):7-13. https://doi.org/10.5281/zenodo.11471554
Wang CY, Sun ZN, Wang MX, Zhang C. SIRT1 mediates salidroside-elicited protective effects against MPP+ -induced apoptosis and oxidative stress in SH-SY5Y cells: involvement in suppressing MAPK pathways. Cell Biol Int. 2018;42(1):84-94. https://doi.org/10.1002/cbin.10864
Pirunkaset E, Boonyarat C, Maneenet J, et al. Effect of Diacetylcurcumin Manganese Complex on Rotenone-Induced Oxidative Stress, Mitochondria Dysfunction, and Inflammation in the SH-SY5Y Parkinson's Disease Cell Model. Molecules. 2024;29(5):957. https://doi.org/10.3390/molecules29050957
Çinar R, Ahlatci A. TRPM2 channel contribution to CdCI2-related neurotoxicity in SH-SY5Y cells: Protective role of selenium: Effect of Se in CdCI2-related neurotoxicity. Neuro-Cell Mol Res. 2024;1(2):40-46. https://doi.org/10.5281/zenodo.13623107
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