TRPV1 channel-mediated apoptotic effects of Liquidambar Orientalis Miller balsam on gastric cancer cell line
Liquidambar Orientalis Miller Gum induces apoptosis in gastric cancer cells
DOI:
https://doi.org/10.5281/zenodo.14557674Keywords:
Liquidambar orientalis, Balsam, TRPV1, Gastric cancer, Apoptosis.Abstract
TRP channels play important roles in various cellular functions, such as proliferation, migration, invasion, and the ability to induce apoptosis in digestive system cancers. Liquidambar orientalis Miller Balsam (resin) is used in the treatment of skin problems, peptic ulcers and parasitic infections, as an antiseptic, as well as a supportive therapy for the treatment of some digestive tract cancers. In our study, we aimed to investigate the TRPV1 channel-mediated apoptotic effects of Liquidambar orientalis Miller balsam on HGC-27 Human Gastric Cancer Cell line.
HGC-27 cells were cultured and divided into three main groups as control, Liquidambar orientalis (balsam) and Liquidambar orientalis+capsazepine. The cells were incubated with Liquidambar orientalis along with a TRPV1 channel inhibitor and stimulator together. The effects of Liquidambar orientalis Miller balsam via TRPV1 channels were investigated in relation to apoptosis, mitochondrial depolarization, ROS and caspase-3 & -9 enzyme activations.
The levels of apoptosis in the Liquidambar orientalis group were significantly higher in cancer cells compared to the control group. TRPV1 channel stimulator administration resulted in significantly increased apoptosis, ROS, caspase-3 and -9 levels in the Liquidambar orientalis group compared to the Liquidambar orientalis+capsazepine group.
The apoptotic effects of Liquidambar orientalis Miiler balsam on gastric cancer cells have been shown to be associated with increased activation of TRPV1 channels due to increased intracellular reactive oxygen species.
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References
Rawla P, Barsouk A. Epidemiology of gastric cancer: global trends, risk factors and prevention. Gastroenterology Review. 2019;14(1):26–38. https://doi.org/10.5114/pg.2018.80001
Bray F, Ferlay J, Soerjomataram I, Siegel R. L, Torre L. A, & Jemal A. Global Cancer Statistics GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA: A Cancer Journal for Clinicians. 2018. https://doi.org/10.3322/caac.21492
Liu Y, Shi Y, Han R, Liu C, Qin X, Li P & Gu R Signaling pathways of oxidative stress response: the potential therapeutic targets in gastric cancer. Front. Immunol. 2023;14:1139589. https://doi.org/10.3389/fimmu.2023.1139589
Sagdic O, Ozkan G, Ozcan M, Ozcelik S. A Study on Inhibitory Effects of Sigla Tree (Liquidambar orientalis Mill. var. orientalis) Storax Against Several Bacteria. Phytother. Res. 2005;19;549–551. https://doi.org/10.1002/ptr.1654
Cetinkaya S, Cinar Ayan I, Suntar I, Dursun HG. The Phytochemical Profile and Biological Activity of Liquidambar orientalis Mill. var. orientalis via NF-κB and Apoptotic Pathways in Human Colorectal Cancer. Nutrition and Cancer, 2021;1-15. https://doi.org/10.1080/01635581.2021.1952455
Atmaca H, Camli Pulat C, Cittan M. Liquidambar orientalis Mill. gum extract induces autophagy via PI3K/Akt/mTOR signaling pathway in prostate cancer cells. International Journal of Environmental Health Research, 2020;1–9. https://doi.org/10.1080/09603123.2020.1818187
Zitt C, Halaszovich CR, Lückhoff A. The TRP family of cation channels: probing and advancing the concepts on receptor-activated calcium entry. Prog Neurobiol. 2002;66(4):243-64. https://doi.org/10.1016/s0301-0082(02)00002-3
Steinberg X, Lespay-Rebolledo C, Brauchi S. A structural view of ligand-dependent activation in thermoTRP channels. Front Physiol. 2014;5:171. https://doi.org/10.3389/fphys.2014.00171
Alsalem M, Millns P, Altarifi A, El-Salem K, Chapman V, Kendall DA. Anti-nociceptive and desensitizing effects of olvanil on capsaicin-induced thermal hyperalgesia in the rat. BMC Pharmacol Toxicol. 2016;17(1):31. https://doi.org/10.1186/s40360-016-0074-9
Pan SY, Zhou SF, Gao SH, Yu ZL, Zhang SF, Tang MK, Ko KM. New Perspectives on How to Discover Drugs from Herbal Medicines: CAM’s Outstanding Contribution to Modern Therapeutics. Evidence-Based Complementary and Alternative Medicine, 2013;1–25. https://doi.org/10.1155/2013/62737
Atmaca H, Camli Pulat C, Cittan M. Liquidambar orientalis Mill. gum extract induces autophagy via PI3K/Akt/mTOR signaling pathway in prostate cancer cells. Int J Environ Health Res. 2022;32(5):1011-1019. https://doi.org/10.1080/09603123.2020.1818187
Uğuz AC, Naziroğlu M, Espino J, et al. Selenium modulates oxidative stress-induced cell apoptosis in human myeloid HL-60 cells through regulation of calcium release and caspase-3 and -9 activities. J Membr Biol. 2009;232(1-3):15-23. https://doi.org/10.1007/s00232-009-9212-2
Martinez NA, Ayala AM, Martinez M, Martinez-Rivera FJ, Miranda JD, Silva WI. Caveolin-1 Regulates the P2Y2 Receptor Signaling in Human 1321N1 Astrocytoma Cells. J Biol Chem. 2016;291(23):12208-12222. https://doi.org/10.1074/jbc.M116.730226
Öz A, Çelik Ö. Curcumin inhibits oxidative stress-induced TRPM2 channel activation, calcium ion entry and apoptosis values in SH-SY5Y neuroblastoma cells: Involvement of transfection procedure. Mol Membr Biol. 2016;33(3-5):76-88. https://doi.org/10.1080/09687688.2017.1318224
Uguz AC, Cig B, Espino J, et al. Melatonin potentiates chemotherapy-induced cytotoxicity and apoptosis in rat pancreatic tumor cells. J Pineal Res. 2012;53(1):91-98. https://doi.org/10.1111/j.1600-079X.2012.00974.x
Özbek AG., Ersus Bilek S. Properties and usage of Liquidambar orientalis. Clin Nutr Metab, 2018;(5)1-3 https://doi.org/10.15761/CNM.1000111
Duran T, Tuncer Z. Investigation of Cytotoxic and Apoptotic Effects of Styrax Liquidus Obtained From Liquidambar orientalis Miller (Hamamelidaceae) on HEp-2 Cancer Cell with Caspase Pathway. Eurasian J Med. 2023;55(3):185-191. https://doi.org/10.5152/eurasianjmed.2023.23130
Mutlu D, Gunal B, Secme M, Bozbeyoglu Kart NN, Abban Mete G, Mercan Dogan N, Semiz G, Melahat Fenkci S and Arslan Ş. Biological activities of Liquidambar orientalis: antibiofilm, cytotoxicity, apoptosis, and miRNA expressions. Botanica Serbica, 2024;48(1):17–26. https://doi.org/10.2298/BOTSERB2401017M
Piciu F, Balas M, Badea MA, Cucu D. TRP Channels in Tumoral Processes Mediated by Oxidative Stress and Inflammation. Antioxidants (Basel). 2023;12(7):1327. https://doi.org/10.3390/antiox12071327
Banner KH, Igney F, Poll C. TRP channels: emerging targets for respiratory disease. Pharmacol Ther. 2011;130(3):371-84. https://doi.org/10.1016/j.pharmthera.2011.03.005
Zhong T, Zhang W, Guo H, Pan X, Chen X, He Q, Yang B, Ding L. The regulatory and modulatory roles of TRP family channels in malignant tumors and relevant therapeutic strategies. Acta Pharm Sin B. 2022;12(4):1761-1780. https://doi.org/10.1016/j.apsb.2021.11.001